Demonstration of CLIP-170 and restin expression by differential PCR.

PCR protocols distinguishing between restin and CLIP-170 either because of length differences of their products (A) or by amplifying only restin, but not CLIP-170 (B), reveal that both variants are weakly expressed in PBMCs (left gel) but are strongly expressed in DCs (monocytes after 90 hours of culture in IL-4 and GM-CSF; right gel).