Western blot analysis of the kinetics of CLIP-170/restin expression during the stimulation of peripheral blood B and T cells.

CLIP-170/restin protein is not detectable using a polyclonal rabbit anti–CLIP-170/restin antiserum in freshly isolated B cells, but it appears after 4 days of culture in IL-4– and CD40L-supplemented medium, whereas CD3 and CD28 activated T cells remain negative. Lane 1, fresh B cells; 2, B cells after 2 days of stimulation with IL4 + CD40 L; 3, B cells after 4 days of stimulation with IL4 + CD40 L; 4, B cells after 10 days of stimulation with IL4 + CD40 L; 5, Hodgkin-derived cell line L540; 6, fresh T cells; 7, T cells 2 days after stimulation with anti-CD3 and anti-CD28; 8, T cells 4 days after stimulation with anti-CD3 and anti-CD28; and 9, T cells 10 days after stimulation with anti-CD3 and anti-CD28. The first and last lanes represent molecular weight markers.