Western blot analysis of the kinetics of CLIP-170/restin expression during the differentiation of adherent monocytes into dendritic cells.

CLIP-170/restin protein is not detectable using a polyclonal rabbit anti–CLIP-170/restin antiserum in freshly isolated monocytes, but it appears after 72 hours of culture in IL-4– and GM-CSF–supplemented medium and reaches a maximum at day 5.