Fig. 3.
Donor cell distributions in nonablated mice after tail vein infusion or direct femur injection.
PKH26-labeled Lin− cells (5 × 105) were infused via tail vein or injected into the cavity of one of the femurs of untreated mice (3 mice per data point; SD was too small to be shown in the figure). At different times (5 minutes, 1 hour, 3 hours, 20 hours, and 50 hours) mice were killed, and the percentages of donor (PKH26-positive) cells in blood, BM, and spleen were determined by FACS analysis. Black lines represent data from intravenous (IV) injection, and red lines represent data from intrafemoral (IF) injection.
