Sufficiency of NUP98-HOXA9 andBCR-ABL for full transformation of mouse bone marrow cells.

(A) Top 4 rows: Southern blot analyses of DNA isolated from the bone marrow (B) and spleen (S) of recipients of NUP98-HOXA9 plusBCR-ABL (left) or HOXA9- plusBCR-ABL–transduced cells (right). Mice were killed when acute leukemia was apparent (ie, at 7 and 9 days after transplantation, respectively). DNA was digested with the indicated restriction enzyme (RE; Figure 1A shows a schematic representation of the integrated provirus). Note the smears in the 3rd and 4th rows (from top) indicating the polyclonal nature of the different leukemias. Bottom 4 rows: Northern blot analyses of RNA isolated for the same mice and hybridized to probes specific to BCR-ABL (BCR)and HOXA9 as indicated. Exposure times were 12 hours(BCR-ABL) and 4 days (HOXA9). (B) Southern blot analyses of DNA isolated from the bone marrow (B) and spleen (S) of recipients of HOXA9-transduced (left) orNUP98-HOXA9–transduced (right) cells killed when acute leukemia was apparent. For HOXA9, DNA was digested withKpnI (for proviral integrity) and with BglII (for clonal analysis). For NUP98-HOXA9, DNA was digested withXbaI and BamHI to test proviral integrity and clonal analysis, respectively. EGFP and RFP probes were used to hybridize DNA isolated from HOXA9- andNUP98-HOXA9–induced leukemias, respectively. Minus signs are as indicated in Figure 1. *Indicates data not available.