Overexpression of Cdc25A^128His or Cdc25A^128Gln decreases the ability of bone marrow cells to form BFU-E.

Bone marrow cells were infected with retroviruses expressing Cdc25A^128His, Cdc25A^128Gln, or vector control and were plated in methylcellulose media containing EPO, SCF, and G418 to select for infected cells. Colonies were scored 7 days after plating. (A) Overexpression of Cdc25A^128His or Cdc25A^128Gln significantly decreases the number of BFU-E compared with vector control. *P < .01; **P < .001. (B) Overexpression of Cdc25A^128Gln significantly decreases the number of CFU-C. All non–BFU-E colonies were scored as CFU-C. **P < .001. Each bar represents the average of 3 independent experiments.