Linkage mapping of Cdc25A on mouse chromosome 9 and comparison of the segregation of BFU-E cell-cycle status and the 2 alleles of

Cdc25A in inbred and congenic mouse strains. (A) Genetic linkage map of the region of mouse chromosome 9 containingFv2/Stk and Cdc25A. Map distances between adjacent loci are given in cM at the left. The centromere is at the top. The linkage map was generated using a C3HheB/FeJ × C57BL/6J)F1 × C57BL/6J backcross panel of 425 animals.10 The linkage map was constructed using MAPMAKER/EXP 3.0.57 (B) Segregation of BFU-E cell-cycle status and the Cdc25A^128His and Cdc25A^128Gln alleles in inbred and congenic strains. Inbred and congenic strains were scored for the Cdc25A alleles by PCR. Results are presented in the left-side boxes. BFU-E cycling status of these strains has been determined by resistance to [³H]thymidine or hydroxyurea and is shown in the right-side boxes. At the top are strains shown previously to beFv2^rr, and at the bottom are strains shown previously to be Fv2^ss. For cell cycling status, strains marked 1 were analyzed by Suzuki and Axelrad7 and strains marked 2 were investigated in this report. B10^s.C indicates B10.C-H7^b(47N)/Sn; B6^s.C, B6.C-H7^b/By.