Fig. 1.
Fig. 1. Mapping of mouse α-globin gene locus. / Panel A shows a map of the mouse α-globin gene cluster, showing the positions of the genes and the regulatory element (HS −26) lying within an intron of the C16orf35 gene. The structures of the targeting vector, the floxed allele after homologous recombination, and the KO allele after expression of Cre recombinase are also shown. The positions of the 5′ and 3′ probes used for mapping are indicated as small black boxes. The band sizes using AflII (A) andBamHI (B) digestion are indicated. Panel B shows a Southern blot analysis of AflII- or BamHI-digested DNA from a WT mouse and heterozygotes for the floxed and KO alleles. The positions of markers (SJ5000, Amersham Pharmacia, Little Chalfont, United Kingdom) are shown along side the gels.

Mapping of mouse α-globin gene locus.

Panel A shows a map of the mouse α-globin gene cluster, showing the positions of the genes and the regulatory element (HS −26) lying within an intron of the C16orf35 gene. The structures of the targeting vector, the floxed allele after homologous recombination, and the KO allele after expression of Cre recombinase are also shown. The positions of the 5′ and 3′ probes used for mapping are indicated as small black boxes. The band sizes using AflII (A) andBamHI (B) digestion are indicated. Panel B shows a Southern blot analysis of AflII- or BamHI-digested DNA from a WT mouse and heterozygotes for the floxed and KO alleles. The positions of markers (SJ5000, Amersham Pharmacia, Little Chalfont, United Kingdom) are shown along side the gels.

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