Integrin-dependent adhesion of uPAR-transfected BAF3 cells.

BAF3 cells were transfected with various amounts of uPAR cDNA and analyzed by flow cytometry using mAb anti-uPAR R4. Adhesion to immobilized mouse ICAM-1–Fc of 5 BAF3 cell lines expressing no uPAR (mean immunofluorescence, 4) or different levels of uPAR (mean immunofluorescence, 50, 85, 103, and 164) was studied. Photomicrographs show results from 1 experiment representative of 5 comparable independent experiments.