Colocalization of DOCK2 with CrkL and F-actin in the hematopoietic cell line.

Jurkat cells were transfected with expression vectors of both CrkL and DOCK2 (panels Aiv-v), of DOCK2 (panels Biv-v), or vector alone (panels Ai-ii,Biv-v), and at 24 hours after transfection, cells were plated on fibronectin-coated slides and fixed with paraformaldehyde. Localization of CrkL and DOCK were analyzed by anti-CrkL (panels Aii,v) and anti-Flag Abs (panels Ai,iv,Bii,v), respectively. F-actin was visualized by phalloidin conjugated with Alexa-594 (panels Bi,iv). Cells were observed with laser scanning confocal microscopy, and merged images are displayed (panels Aiii,vi,Biii,vi). Original magnification × 400.