Nf1 homozygosity results in an elevated number of mature T cells in the spleen without altering differentiation.

(A) Comparison of CD4 and CD8 SP T-cell numbers between WT→RAG2^−/− andNf1^−/−→RAG2^−/− spleen. Bars represent the absolute numbers of indicated T-cell subsets (± SEM), n = 7 and * P < .0007. (B) Immunocytometric analysis of splenocytes from RAG2^−/− (did not receive transplants), WT→RAG2^−/−, andNf1^−/−→RAG2^−/− mice. Splenocytes were stained with fluorochrome-conjugated antibodies directed against the indicated cell surface proteins and analyzed by flow cytometry. Data are representative of 3 independent experiments with similar results.