Nf1 homozygosity results in splenic hyperplasia.

(A) Comparison of splenic size between WT and Nf1^−/− mice. RAG2^−/− mice received equal numbers of WT orNf1^−/− fetal liver or bone marrow hematopoietic stem and progenitor cells and were killed 2 to 3 months after transplantation. Shown are representative WT andNf1^−/− spleens from a total of 7 analyzed with similar results. Original magnification, × 10. (B) Quantitative comparison of splenic cellularity in RAG2^−/− mice that received WT andNf1^−/− fetal liver or bone marrow cells. Single cell suspensions from WT and Nf1^−/−spleens were prepared and counted. Bars represent the absolute numbers of splenocytes (± SEM) from a total of 7 mice of each type. * P < .0005.