Tax induces constitutive JNK activation and p-c-Jun up-regulation in peripheral T cells and in HTLV-1–transformed cell line MT2.

(A) Peripheral T cells were stimulated with anti-CD3 (100 ng/mL), and PBMCs were stimulated with PHA/IL-2 and were harvested at the indicated times. Lysates were subjected to immunoblot probed with an anti–p-c-Jun antibody. (B) p-c-Jun and Tax expression were detected by an anti–p-c-Jun and anti-Tax antibodies using immunoblot analysis with total lysates of Jurkat or MT2 cell lines, ΔU3CMVTax-transduced or -untransduced PBMCs.