Fig. 4.
Fig. 4. Cytotoxic granule constituents and cytokine production. / (A) TCRγδ− CD16− PBMNCs were stained with anti-CD8β mAbs recognized by FITC-conjugated goat-antimouse antibodies, RPE-Cy5–conjugated anti-CD8α, and PE-conjugated antiperforin, antigranzyme B, or anti–TIA-1 mAbs. (B) After the stimulation with PMA and A23187 in the presence of monensin, TCRγδ−CD16− PBMNCs were stained with PE-conjugated anti-CD8β, RPE-Cy5–conjugated anti-CD8α, and FITC-conjugated anti–IL-2 or anti–IFN-γ mAbs. CD8α gated cells are displayed.

Cytotoxic granule constituents and cytokine production.

(A) TCRγδ− CD16− PBMNCs were stained with anti-CD8β mAbs recognized by FITC-conjugated goat-antimouse antibodies, RPE-Cy5–conjugated anti-CD8α, and PE-conjugated antiperforin, antigranzyme B, or anti–TIA-1 mAbs. (B) After the stimulation with PMA and A23187 in the presence of monensin, TCRγδ−CD16− PBMNCs were stained with PE-conjugated anti-CD8β, RPE-Cy5–conjugated anti-CD8α, and FITC-conjugated anti–IL-2 or anti–IFN-γ mAbs. CD8α gated cells are displayed.

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