Fig. 7.
![Fig. 7. CD34+-derived int-DCs mature in response to LPS/CD40L. / CD34+-derived int-DCs were stimulated for 24 hours with 1 μg/mL LPS (int-DC + LPS) and then for 48 hours with either 10 μg/mL anti-CD40 (G-28.5, American Type Culture Collection [ATCC]) (int-DC + LPS + αCD40) (A) or CD40L on transfected L-cells (int-DC + LPS + CD40L) (B). (A) Cell morphology as seen by microscopy. (B) FACS-measured antigenic expression, using the same Ab as in Figure 2. Percentages of labeled cells and the MFI for CD40 expression are indicated. White histograms represent isotype controls, and specific labeling is shown in gray. The data are representative of 5 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/10/10.1182_blood-2002-01-0312/4/h82223386007.jpeg?Expires=1722700053&Signature=eNhYiz2sAJIDegJ-CYsH18AHDY~8jv-HPtoXjL9BjpP8ymDSkKKvqN6OIxBPXBCxKp5rbMmu1-zNeSp6N0wpMmIy5x3XZkv4G7YDSjTxZG-qQpkg7PkUr162inxEacXJsLRZ9ooMYf96R5h2Ut14XkvDkg9q4eWr8JGwm2OGsibe6XFLqPaPkkgveQOtynHZlpUzpsflToFZ~R4VI3CvmMR~5y483aeMSMuKjBq-8puHSWXeB-bRQ04Jor6IzYQE4djCOh-TPFGi-TsBVIO~yH~4ByEQwv2jmT7KSmGDqkSo3nQRyLHOw~dVqSusfdfpXyxaKimS4wTNEaS1hECFjg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD34+-derived int-DCs mature in response to LPS/CD40L.
CD34+-derived int-DCs were stimulated for 24 hours with 1 μg/mL LPS (int-DC + LPS) and then for 48 hours with either 10 μg/mL anti-CD40 (G-28.5, American Type Culture Collection [ATCC]) (int-DC + LPS + αCD40) (A) or CD40L on transfected L-cells (int-DC + LPS + CD40L) (B). (A) Cell morphology as seen by microscopy. (B) FACS-measured antigenic expression, using the same Ab as in Figure 2. Percentages of labeled cells and the MFI for CD40 expression are indicated. White histograms represent isotype controls, and specific labeling is shown in gray. The data are representative of 5 independent experiments.
