CD34⁺-derived int-DCs efficiently capture antigen.

(A) May-Grünwald-Giemsa staining and anti–HLA DR labeling (revealed in DAB) of cytospun CD34⁺-derived int-DCs (panels Ai and Aii, original magnification, × 400). CD34⁺-derived int-DCs were incubated for 2 hours with heat-killed yeast and then thoroughly washed. Compare with CD34⁺-derived int-DCs in culture without yeast (panels Aiii and Aiv, original magnification, × 350). (B) Myc409, a recombinant Mycobacterium bovis BCG strain expressing GFP, was added for 4 hours to CD34⁺-derived int-DCs at an MOI of 1 or 10. Capture of Myc409 was measured by flow cytometry. The data are representative of 2 independent experiments.