CD34⁺-derived int-DC longevity is mediated by constitutive TRANCE-RANK ligation.

(A) CD34⁺-derived int-DCs were cultured for 12 days in the presence of either human IgG1 or RANK-Fc. Cell viability was determined by trypan blue exclusion of dead cells. The results are expressed as the means of 5 independent experiments with SD of the data. (B) IgG1- or RANK-Fc–treated CD34⁺-derived int-DCs were stained for intracellular Bcl-2, and the percentage of Bcl-2–expressing cells is indicated. Results are representative of 5 independent experiments.