TRANCE and RANK are novel int-DC antigens.

CD14⁺ DCs purified from human skin (A) and liver (B) were analyzed for TRANCE and RANK expression by FACS. Percentage of positive cells is indicated. (C) Generation of macrophage-related cells from CD34⁺ progenitors (CD34⁺-derived int-DCs). Cord blood CD34⁺ cells were cultured for 6 days in SCF, GM-CSF, and TNFα, yielding a CD14⁺CD1a⁻ population, which was FACS-sorted and grown in M-CSF for 4-6 days.25(D) Antigen expression of CD34⁺-derived int-DCs. The SSC/FSC scattergram profile is shown, and no electronic gates were set. The expression of surface markers was determined by flow cytometry using specific antibodies. White histograms represent isotype controls, and specific labeling is shown in gray.