Analysis of the liver of splenectomized mice.

(A) Liver sections of 2 wild-type (i,iii) and 2 HpHx dKO (ii,iv) mice one month (i-ii) and 2 months (iii-iv) after splenectomy, stained with hematoxylin and eosin (i-ii) and with the Masson trichrome reaction (iii-iv). Note extensive necrotic areas in the liver of HpHx dKO mouse one month after splenectomy (ii; arrows) and massive fibrosis one month later (iv). For all panels, scale bar = 200 μm. (B) Slot blot of total RNA from wild-type (on the left) and HpHx dKO mice (on the right) under basal conditions and one and 2 months after splenectomy, assayed for SAA3 and β-actin expression. Note the strong expression of SAA3 only in HpHx dKO mice 2 months after splenectomy.