No immune reaction against the injected antibodies was observed.

Anti-OB-R (a) and irrelevant (anti–human casein) mAbs (b) were diluted 5-fold from the treatment concentration of 1 μg/mL, and 5 μL of the different dilutions were applied on nitrocellulose membranes. Rat plasma was used as positive control. The membranes were incubated with pooled plasma from 3 BN rats given the irrelevant mAb (1), or from 3 BNML rats given anti–OB-R (2), or from 3 leukemic fa/farats given anti-Ob-R (3), or buffer as negative control (4).