The 13A2 mAb is specific for human KLRG1.

(A) COS7 cells were transiently transfected with empty expression vector (mock) or with expression vector containing human KLRG1 cDNA. Afterward, cells were stained with the indicated mAb (shaded histograms) and analyzed by flow cytometry. Open histograms represent negative controls. To determine cross-reactivity of the 13A2 mAb, KLRG1⁺ RBL-2H3 cells from rat and KLRG1⁺ CD8 T cells from mice were examined in a similar fashion, including mAb G63 and 2F1 specific for rat and mouse KLRG1, respectively, as positive controls. (B) Immunoprecipitation of KLRG1. Detergent extracts of surface iodinated human PBL were incubated with beads coupled with mAb 13A2, and bound material was analyzed in 12.5% SDS-PAGE under nonreducing conditions.