Figure 2.
Visualization of thrombus formation by a HIT-like monoclonal antibody. Confocal microscopy images of fixed thrombi formed in human whole blood perfused with a control antibody (RTO) or a HIT-like antibody (KKO), and PF4. Platelet aggregates are shown in green, fibrin fibers visualized by adding of Alexa 647-labeled fibrinogen are purple, and white blood cells are shown in cyan (overlap of blue nuclear dye is Hoechst and green is calcein AM). The attachment points of fibrin to platelets are white because of the superposition of purple and green colors. Reprinted from Tutweiler et al10 with permission.
