Effect of BCL2L1 overexpression on cell differentiation, proliferation, and survival. (A) Effect of BCL2L1 ectopic expression on CD71 and CD235 expression (n = 3; upper panel). Representative flow cytometric analyses of CD71 and GlyA (lower panels). (B) Effect of BCL2L1 ectopic expression on cell enucleation during differentiation (n = 3; upper panel). Representative images of Wright-Giemsa staining (lower panels, original magnification ×40). (C) Effect of BCL2L1 ectopic expression on erythroid progenitor cell proliferation. The BCL2L1- or vector only–expressing CD34⁺ cells were cultured in StemSpan SFEM II medium, and cell numbers were counted at days 1, 3, and 6 (n = 3). (D) Effect of BCL2L1 ectopic expression on erythroid progenitor cell survival during the differentiation. BCL2L1- or vector only–expressing CD34⁺ cells were cultured in differentiation medium. Cells stained with trypan blue were counted and the percentages of live cells are shown (n = 4). **P < .01.