BCL2L1 regulates HBG gene expression and F-cell production in erythroid progenitors derived from adult CD34⁺cells. (A) BCL2L1 ectopic expression upregulated HBG gene expression. BCL2L1 and HBG mRNA were normalized to β-actin (n = 3). (B) BCL2L1 predominantly regulated HBG, with a minor effect on HBB and HBA. BCL2L1, HBG, HBB, and HBA mRNA in BCL2L1-overexpressed and vector-expressed cells was normalized to β-actin, and the fold changes in BCL2L1, HBG, HBB, and HBA mRNA are shown (n = 3). (C) BCL2L1 ectopic expression increased F-cell production (n = 3; upper panel). Representative flow cytometric analyses of F cells (lower panels). (D) BCL2L1 ectopic expression increased F-cell production from the CD235 population (n = 3; upper panel). Representative flow cytometric analyses of HbF and CD235 double staining (lower panels). *P < .05, **P < .01.