Identification of differentially expressed genes in CD14⁺cells from CMML patients and old and young controls. Highly purified CD14⁺ monocytes were subjected to RNA sequencing. (A) Differentially expressed genes in CMML CD14⁺ monocytes compared with old healthy controls were graphed according to fold change and FDR-adjusted P value (volcano plot). (B) Differentially expressed genes in CD14⁺ monocytes of healthy young controls compared with healthy old controls were graphed according to fold change and FDR-adjusted P value (volcano plot). (C) Heat map depicting the top 12 most significantly upregulated genes and the top 12 most significantly downregulated genes in the CMML vs old controls comparison clustered by rlog values. (D) Heat map depicting the top 12 most significantly upregulated genes and the top 12 most significantly downregulated genes in the young controls vs old controls comparison clustered by rlog values.