SR1 proT cells can differentiate into heterogeneous T-cell types. (A) t-distributed stochastic neighbor embedding (tSNE) plot showing 7 cell clusters from splenic CD3⁺ T cells in naive CD7⁺ or SR1 CD7⁺ cell–engrafted mice. (B) Analysis of CD4 and CD8A gene expression among CD3⁺ T cells derived from both naive CD7⁺ or SR1 CD7⁺ cell–engrafted mice. (C) Heatmap showing expression of T-cell function–associated genes in each cluster. Results are filtered based on CD4 or CD8A expression, as indicated. Black boxes highlight the prominent patterns defining known T-cell subtypes (n = 3 mice pooled for each group). Treg, regulatory T cell.