Effect of high-level ET3 expression on erythropoiesis. (A) Blood cell counts in hCD46-transgenic mice before treatment and at week 24 after in vivo HSC transduction. (B) Hematological parameters. Statistical analysis was performed using 2-way ANOVA. The differences between the 2 groups were not significant. (C) Representative blood smears before treatment and at week 24 after treatment in a mouse that expressed high-level ET3. Nuclei of white blood cells (WBCs) stain purple. Scale bars, 20 μm. (D) Cellular bone marrow composition in control and treated mice euthanized at week 24. Shown is the percentage of lineage marker-positive cells (Ter119⁺, CD3⁺, CD19⁺, and Gr-1⁺ cells). The differences between the 2 groups were not significant. BA, basophils; EO, eosinophils; Hb, hemoglobin; HCT, hematocrit; LY, lymphocytes; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; MCV, mean corpuscular volume; NE, neutrophils; RDW, red cell distribution width.