Lepr-cre⁺cells divide BM MSCs into 2 functionally distinct subpopulations. (A) Lepr-cre⁺;iDTR^ki/wt mice were injected with DT for 3 consecutive days and the effect on niche cells was analyzed on day 4. Control (Ctrl), n = 10; Cre⁺ iDTR⁺, n = 10; pool of 3 experiments. (B) In vitro differentiation of sorter-purified indicated cells using adipogenic or osteogenic culture conditions. Scale bars, 100 μm. Lepr^+/−, n = 2; OP, n = 4; Endo, n = 5. ns, not significant.