Alterations in the human platelet transcriptome during clinical sepsis are similar in murine platelets during experimental sepsis. (A) Filtering strategy for identifying orthologous and correlative human and mouse platelet transcriptome changes identified by RNA-seq. Of all transcripts identified by RNA-seq in human or murine platelets, we first restricted the analyses to transcripts with >0.3 FPKM. We next retained only those transcripts orthologous between the 2 species (n = 6755). (B) Correlation plot of the expression of conserved platelet transcripts in untreated control mice (n = 4) and healthy human donors (n = 5). (C) Correlation plot of the expression of conserved platelet transcripts in CLP-treated mice (n = 3, pooled platelets) and septic patients (n = 5). (D) Correlation plot of the change in expression (fold-change) of orthologous, significantly (P < .05) differentially expressed transcripts in both mouse and human platelets under septic conditions.