The influence of ADC-mediated preconditioning on blood cell lineage reconstitution after HSCT. Sca-1 cells were isolated from CD45.2/CAG-GFP–transgenic (CD45.2/GFP^Tg) mice and transplanted into CD45.2/FVIII^null mice under (CD45.2+CD117)-ADC preconditioning. Platelets were stained with CD41 antibody. Leukocytes were stained with CD45.2, CD4, CD8, and B220 antibodies, and donor-derived GFP⁺ chimerism was analyzed by flow cytometry at various time points after HSCT. (A) Schematic diagram of experimental design for GFP^Tg HSCT using (CD45.2+CD117)-ADC preconditioning. (B) Platelet chimerism. (C) Leukocyte chimerism. (D) CD4 T cell chimerism. (E) CD8 T cell chimerism. (F) B-cell chimerism. (G) Myeloid chimerism. (H) Sustained high levels of platelet chimerism in primary and secondary transplant recipients. (I) Sustained leukocyte chimerism in primary and secondary transplant recipients. These results demonstrate that (CD45.2+CD117)-ADC preconditioning is favorable for platelet lineage reconstitution after HSCT.