NETs play a role in adhesion induction, and depletion of NETs and neutrophils result in adhesion prevention. (A) Percentage of neutrophils in peripheral blood 6, 12, 24, and 48 hours following anti–Gr-1 administration. (B) Adhesion scoring of adhesion-induced mice treated with PBS (n = 63), Gr-1 alone (n = 4), MCP-1 alone (n = 5), and Gr-1 combined with MCP-1 (n = 14). (C) Single and composite immunofluorescence staining for H3Cit and Hoescht in adhesion tissue 7 days following adhesion induction. White arrows denote areas highly concentrated for nuclear stain and H3Cit suggestive of NETs. (D) Single and composite immunofluorescence staining for Gr-1 and H3Cit in wild-type/RFP⁺ parabiotic mice. (C-D) Immunofluorescence stain. Scale bar, 100 µm. (E) Adhesion scoring of adhesion-induced mice treated with intraperitoneal injections of PBS (n = 4) or DNase I (n = 5) or osmotic pumps filled with PBS (n = 5) or DNase I (n = 5). NS, not significant.