The mesothelium recruits neutrophils and macrophages through during adhesion induction. (A) Heatmap of immune active proteins from RNA sequencing of purified surface mesothelium immediately after and 6, 12, and 24 hours after button placement and clustered by gene expression. (B) Heatmap of Luminex multiplex assays of key immune active proteins. (C) Characterization of myeloid population from 0 to 96 hours following adhesion formation (top). Absolute numbers of macrophages (blue) and neutrophils (red) were analyzed at each time point. (Bottom) Percentage of F4/80hi and Gr-1hi cells after 24 hours in untreated, sham surgery, or adhesion-induced mice. (D) Single and composite immunofluorescence staining for Gr-1 of adhesions from wild-type mice of a wild-type/RFP⁺ parabionts pair. Immunofluorescence stain. Magnification ×20. Arrows denote RFP⁺Gr-1⁺ cells. CSF, colony-stimulating factor; FPKM, fragments per kilobase of transcript per million mapped reads; GMCSF, granulocyte macrophage colony-stimulating factor; GSCF, granulocyte colony-stimulating factor; IFN, interferon; IL, interleukin; IP, interferon γ–induced protein; LIF, leukemia inhibitory factor; LIX, CXCL5; max, maximum; MCSF, macrophage colony-stimulating factor; min, minimum; MIP, macrophage inflammatory protein; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.