Figure 2.
Gene expression and signaling pathways analysis in HSPCs after platelet depletion. (A) GSEA of upregulated genes in CD41+ and CD41− HSCs, with or without platelet depletion. (B) Heat map depicting Mk-specific genes in CD41+ and CD41− HSCs, with or without platelet depletion. (C) Representative graphs showing pStat5 and pErk1/2 levels in HSCs, MPP2, PreMegs, MPP3/4, and common myeloid progenitor–granulocyte macrophage progenitor (CMP-GMP) cells at 2 hours after platelet depletion (data combined from 4 independent experiments, n = 8). Ctrl. in black, Depl. in red. (D) Quantification of pErk1/2 and pStat5 levels in HSCs, MPP2, MPP3/4, PreMegs, and CMP-GMP cells at 2 hours after platelet depletion (data combined from 3 independent experiments, per data point Ctrl. n = 2; Depl. n = 2, connecting lines show samples from the same experiment). Data are represented as mean for each experiment. The P values were calculated by 2-tailed paired Student t tests.

Gene expression and signaling pathways analysis in HSPCs after platelet depletion. (A) GSEA of upregulated genes in CD41+ and CD41− HSCs, with or without platelet depletion. (B) Heat map depicting Mk-specific genes in CD41+ and CD41− HSCs, with or without platelet depletion. (C) Representative graphs showing pStat5 and pErk1/2 levels in HSCs, MPP2, PreMegs, MPP3/4, and common myeloid progenitor–granulocyte macrophage progenitor (CMP-GMP) cells at 2 hours after platelet depletion (data combined from 4 independent experiments, n = 8). Ctrl. in black, Depl. in red. (D) Quantification of pErk1/2 and pStat5 levels in HSCs, MPP2, MPP3/4, PreMegs, and CMP-GMP cells at 2 hours after platelet depletion (data combined from 3 independent experiments, per data point Ctrl. n = 2; Depl. n = 2, connecting lines show samples from the same experiment). Data are represented as mean for each experiment. The P values were calculated by 2-tailed paired Student t tests.

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