Genomic profiling of KSHV and EBV miRNAs. (A) Outline of miRNA maturation and the species that can be detected using pre-miRNA–specific primers and SYBR-based real-time QPCR or miRNA-specific primers and TaqMan-based real-time QPCR. (B) Plot of relative copy number of viral miRNA gene loci. Variations in input DNA were adjusted using U6. This yielded dCT_U6, which represents a logarithmic measure of relative gene copy number. Primers are indicated on the horizontal axis. Positive dCT_U6 indicate lower copy number. Colors indicate different cell lines. The JSC-1 and BC-1 cell lines are EBV positive.