Cholesterol sensitivity of CD20 epitopes. Ramos B cells were treated with methyl-β-cyclodextrin to deplete membrane cholesterol before performing indirect staining with a panel of 15 CD20 antibodies (□), or antibodies against CD10, CD19, CD22, or CD45 (■) for comparison. The bars show the mean fluorescence intensity (MFI) of treated cells as a percentage of MFI values from untreated cells. Data are representative of at least 2 tests of each antibody.