c-KIT signals through JAK1 and JAK3 in HMC1.1 cells. HMC1.1 cells were serum-starved overnight and stimulated with 100 ng/mL SCF for 5 minutes. Cell lysates were immunoprecipitated for c-KIT, JAK1, or JAK3. Immunoprecipitates and whole cell extract (WCE) were analyzed by immunoblot for c-KIT, total and phospho JAK1 and JAK3, and β-actin.