Scheme for RNAi functional profiling of AML cells. Thirty-five percent of AML cases exhibit phosphorylated STAT5 without knowledge of specific tyrosine kinases that are dysregulated. To better understand which tyrosine kinases contribute to this disease, we administered siRNA individually targeting each member of the tyrosine kinase family as well as N-RAS, K-RAS and 2 controls (CTRL) into AML cell lines. Cells were plated into culture media and subjected to an MTS assay at day 4 after electroporation for determination of cell viability and proliferation. All absorbance values were normalized to the absorbance values of 2 nonspecific control siRNA molecules.