LMO2 is not activated by globin regulatory elements. A cassette containing the GFP coding sequences under the control of β-globin gene promoter along with the human β-globin LCR was used in the cassette exchange reaction in R-6 cells. Five clones were confirmed as having undergone the predicted exchange. Activation of the LMO2 gene in all 5 clones was minimal, if any, as determined by qRT-PCR (A) or Western blot analysis (B).