Impaired IL-12 production by splenic DCs and lung DCs day 11 after experimental peritonitis. (A) Purified splenic DCs from septic or control mice on day 11 after surgery were stimulated by a series of TLR agonists. Splenic DC mRNA levels of the 2 IL-12 subunits and IL-10 were determined by Taqman. (B) Splenic DC protein levels of IL-12p70 and IL-10 were measured by Bio-Plex. (C) Purified lung DCs from septic or sham mice on day 11 after surgery were stimulated by a series of TLR agonists, and protein levels of IL-12p70 and IL-10 were measured by Bio-Plex. Results are representative of 3 to 5 independent experiments and are expressed as mean plus or minus SEM. *P ≤ .05 compared with cytokine levels measured in splenic DCs from sham mice.