Silencing of Lyn or PAG induces cell death in Jeko-1 (MCL), but not in L428 Hodgkin cells. (A) Viability of L428 and Jeko-1 cells 72 hours after transduction of Lyn or PAG sequences (in Table S1: control Lyn, sequence 3) and siLyn (sequence 2), control PAG (sequence 9), and siPAG (sequence 10), evaluated after ethidium bromide labeling. Statistical analysis was carried out by Student t test (**P < .001; ***P < .0001). (B) Lyn and PAG protein expression was measured by WB with anti-Lyn and anti-PAG MEM255 antibodies. Equivalent loading was verified by anti-Csk WB because the Csk protein is not affected by Lyn or PAG silencing. Intensities of Lyn and PAG bands were measured with ImageQuant. These results are representative of 3 independent silencing experiments.