Phosphorylation of Akt by HRF/TCTP. (A) Purified basophils were stimulated with various doses of HRF/TCTP, and cell lysates were prepared and Western blotted for phospho-Akt as described in “Methods.” This panel shows phosphorylation of Akt in basophils from an HRF/TCTP-R donor (top row; R) or an HRF/TCTP-NR donor (bottom row; NR). Cells were stimulated with anti-IgE (aIgE) as a positive control, buffer as a negative control (“C”), or increasing doses of HRF/TCTP. (B) Percentage of maximum of Akt phosphorylation with increasing doses of HRF/TCTP (●) in 3 HRF/TCTP donors. There is no detectable phosphorylation induced by HRF/TCTP in 3 HRF/TCTP-NR donors (□). The standard error is represented by the error bars.