Lineage switch after FOG-1 expression in progenitor cells. (A) Giemsa staining and FACS analysis on day 14 of E14tg2a ES cells in the presence of IL-3 since day 5. Antibody staining (blue line) and IgG control staining (gray line) were shown. (B) Giemsa staining of day 14 cells derived from TET-FOG-1 ES cells. Differentiation was induced in TET-FOG-1 ES cells without FOG-1 expression until day 8. IL-3 was added from day 5, and FOG-1 was induced on day 8. (C) FACS analysis of day 14-induced cells stained with anti-c-KIT, GR-1, and MAC-1 antibodies. The culture conditions were the same as in (B). (D) The total number of day 14 cells differentiated from TET-FOG-1 ES cells. Error bars represent SEM. (E) Percentage of each type of colony. The day 8 cells induced from TET-FOG-1 ES cells without FOG-1 expression were transferred to methylcellulose media containing IL-3, and FOG-1 expression was induced. Individual colonies were selected and analyzed after 7 days of culture. Bars represent 50 μm.