The effect of down-regulation of PRL-3 expression on migration, proliferation, and cell cycle in INA-6 cells. (A) Transfection of PRL-3 siRNA reduced SDF-1–induced migration in INA-6 cells as compared with transfection with control siRNAs. Wild-type INA-6 cells are cells stimulated with SDF-1 but not transfected. (B) PRL-3 siRNA as well as control siRNAs did not influence the proliferation of INA-6 cells. (C) PRL-3 siRNA did not induce cell-cycle arrest. The percentages of cells in G0/G1, S, and G2M in wild-type INA-6 were 35.2%, 44.9%, and 19.9%, respectively. In transfected cells, the percentages were 32.3%, 44.4%, and 23.4% in siRNA-negative control; 29.7%, 46.2%, and 24.1% in positive control siRNA cyclophilin B; and 32.2%, 46.6%, and 21.3% in siRNA PRL-3, respectively. (D) The down-regulation of the PRL-3 protein was verified on Western blot. Similar results were shown in 3 independent experiments. Error bars represent +1 standard deviation of 5 (migration) and 4 (thymidine incorporation) measurements.