Expression of PRL-3 mRNA in myeloma and nonmyeloma cell lines. The ΔCt between PRL-3 and β-actin for CAG was 8.1 (± 0.58). The relative expression level of PRL-3 to β-actin in the CAG cells, 2^−ΔCt, was arbitrarily set to 1. The relative expression level of other cell lines was normalized to that of CAG cells and was represented by 2^−ΔΔCt, as is illustrated in this figure.