Tumor vessels in Cryab^−/− mice are leaky, partially lack NG-2–positive pericyte coverage, and show signs of apoptosis. (A) Fibrin deposits (brown, indicated by arrows) around tumor vessels in wild-type (wt; left) and Cryab^−/− (right) mice (bar = 100 μM). (B) NG-2–positive pericytes (green) in VEGFR-2 positive vessels (red) in tumors grown in wild-type (top) and Cryab^−/− (bottom) mice (bar = 100 μm). Arrows show vessels covered by NG-2–positive pericytes, and arrowheads show vessels lacking pericyte coverage. (C) CD31-positive tumor vessels (red) in Cryab^−/− mice with partial expression of proapoptotic cleaved caspase-3 (green, arrow) and (*) apoptotic vessel expressing cleaved caspase-3, magnified below (bar = 100 μM). White dotted lines in magnifications indicate the vessel borders.