Bmx targets the 3′UTRs of TNF and IL-6 after LPS stimulation. (A) Schematic representation of the human IL-6 5′ promoter-luciferase-3′UTR (AdIL-6 5′/3′) and IL-6 5′ promoter-luciferase (AdIL-6 5′only) adenoviral constructs. (B) Macrophages were left uninfected or infected with Ad0 and AdBmx before a second round of infections with AdTNF 5′/3′, AdTNF5′, AdIL-6 5′/3′, or AdIL-6 5′ luciferase reporter gene constructs and stimulated with LPS (10 ng/mL) for 4 hours. Relative luciferase units (RLU) for uninfected, Ad0-infected, and AdBmx-infected cells in the absence or presence of TNF reporter viruses (top panel) or IL-6 reporter viruses (bottom panel) after 4 hours of LPS stimulation are shown as mean plus or minus SEM normalized to controls for 4 separate donors. Statistical significance was assessed by Student ttest (*P< .05; **P< .01).