Fas-induced cell death varies with cell type in the presence of Sema3A. (A) Jurkat, HUT78, and HUT78.B1 cells were assayed by flow cytometry for their respective cell surface content of Fas. (B,C) Jurkat, HUT78, or HUT78.B1 cells were stimulated with 50 ng/mL rhFasL, 200 ng/mL CH11, 150 ng/mL APO1-1, and 50 ng/mL TRAIL, in the presence of soluble control IgG or Sema3A-Fc (150 ng/mL) for 7 hours. Cell death was assessed by staining using FITC-annexin V. Data are mean plus or minus SEM of 3 independent experiments (*P < .05, vs none; **P < .05, vs its respective death receptor ligand).