AE increases the mutation frequency in vivo. Mutation frequency of AE and control MIGR1 murine bone marrow cells after 3 months in vivo. Data represent averages of 8 mice (MIGR1) and 7 mice (AE) from 3 separate transductions. The hatched area represents mutations with no change in plasmid size (primarily point mutations); the remainder are translocations or deletions. Error bars represent SEM.