CaMKIV regulates lifespan and Bcl-2 family protein accumulation. CD11c + DCs were positively selected from WT (Camk4^+/+), Camk4^−/−, BCL-2^tg/tg transgenic, and Camk4^−/−/BCL-2^tg/tg hybrid mice cultured in the presence or absence of LPS (10 μg/mL). (A,D) Viability was assayed by trypan blue exclusion at daily intervals. The results represent mean and SD of 6 independent experiments. (B,E) Typical results obtained by immunoblot analysis. Bar graphs show mean (± SD) of the optical density measurements expressed as the ratio between Bcl-2 or Bcl-xL and actin bands (n = 6). (C) Immunoblot shows the typical expression of CaMKIV and hu-Bcl-2 detected in WT (Camk4^+/+), Camk4^−/−, BCL-2^tg/tg transgenic, and Camk4^−/−/BCL-2^tg/tg hybrid mice (lanes 1, 2, 3, and 4, respectively). *P < .01.