CaMKIV is required to link TLR4 signaling with pCREB accumulation. CD11c + DCs were isolated from spleens of WT or Camk4^−/− mice and cultured in the presence or absence of LPS (10 μg/mL) for 1 hour. Whole lysates were separated by SDS-PAGE and immunoblotted with the reported antibodies (pCREB, pAKT, actin, LPS). A typical immunoblot analysis is shown.